hnf4 (ser313) polyclonal antibody Search Results


p hnf4α  (Bioss)
92
Bioss p hnf4α
LOX-1 silencing increased autophagy of HLSECs through <t>AMPK/HNF4α</t> signaling pathway. All groups were treated with 25 mM of glucose for 72 h, except for the normal control group. The cells in the transfection group HG + LV-LOX-1 or HG + LV-CON cells were blocked with AMPK (10 μM), HNF4α (0.6 μM), or autophagy (6 mM) inhibitors. The untreated intact samples were run as a control in each experiment. (A–C) HLSECs were cultured under the above conditions for 72 h, and the mRNA expression levels of LOX-1, Beclin-1, and LC3II in each group were determined by qRT-PCR. (D) The protein expression levels of each group were determined using western blotting, and β-actin was used as a loading control (see original blots in Supplemental Figure 6D). (E–K) Western blotting analyzed the relative protein expression of LOX-1, Beclin-1, LC3II, AMPK, p-AMPK, HNF4α, and p-HNF4α in each group (n = 3 independent experiments). All data are expressed as mean ± SD. from three independent experiments. ∗ P < 0.05, ∗∗∗ P < 0.01 versus the NC group; # P < 0.05, ### P < 0.001 versus HG + LV-CON group; x P < 0.05, xx P ​< 0.05, xxx P < 0.01 versus HG + LV-LOX-1 group.
P Hnf4α, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p hnf4α/product/Bioss
Average 92 stars, based on 1 article reviews
p hnf4α - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


LOX-1 silencing increased autophagy of HLSECs through AMPK/HNF4α signaling pathway. All groups were treated with 25 mM of glucose for 72 h, except for the normal control group. The cells in the transfection group HG + LV-LOX-1 or HG + LV-CON cells were blocked with AMPK (10 μM), HNF4α (0.6 μM), or autophagy (6 mM) inhibitors. The untreated intact samples were run as a control in each experiment. (A–C) HLSECs were cultured under the above conditions for 72 h, and the mRNA expression levels of LOX-1, Beclin-1, and LC3II in each group were determined by qRT-PCR. (D) The protein expression levels of each group were determined using western blotting, and β-actin was used as a loading control (see original blots in Supplemental Figure 6D). (E–K) Western blotting analyzed the relative protein expression of LOX-1, Beclin-1, LC3II, AMPK, p-AMPK, HNF4α, and p-HNF4α in each group (n = 3 independent experiments). All data are expressed as mean ± SD. from three independent experiments. ∗ P < 0.05, ∗∗∗ P < 0.01 versus the NC group; # P < 0.05, ### P < 0.001 versus HG + LV-CON group; x P < 0.05, xx P ​< 0.05, xxx P < 0.01 versus HG + LV-LOX-1 group.

Journal: Heliyon

Article Title: LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs

doi: 10.1016/j.heliyon.2022.e12385

Figure Lengend Snippet: LOX-1 silencing increased autophagy of HLSECs through AMPK/HNF4α signaling pathway. All groups were treated with 25 mM of glucose for 72 h, except for the normal control group. The cells in the transfection group HG + LV-LOX-1 or HG + LV-CON cells were blocked with AMPK (10 μM), HNF4α (0.6 μM), or autophagy (6 mM) inhibitors. The untreated intact samples were run as a control in each experiment. (A–C) HLSECs were cultured under the above conditions for 72 h, and the mRNA expression levels of LOX-1, Beclin-1, and LC3II in each group were determined by qRT-PCR. (D) The protein expression levels of each group were determined using western blotting, and β-actin was used as a loading control (see original blots in Supplemental Figure 6D). (E–K) Western blotting analyzed the relative protein expression of LOX-1, Beclin-1, LC3II, AMPK, p-AMPK, HNF4α, and p-HNF4α in each group (n = 3 independent experiments). All data are expressed as mean ± SD. from three independent experiments. ∗ P < 0.05, ∗∗∗ P < 0.01 versus the NC group; # P < 0.05, ### P < 0.001 versus HG + LV-CON group; x P < 0.05, xx P ​< 0.05, xxx P < 0.01 versus HG + LV-LOX-1 group.

Article Snippet: Antibodies against AMPK (bs-1115R), p-AMPK (bs-14318R), HNF4α (bs-3828R), p-HNF4α (bs-4001R), LC3 (bs-8878R), beclin-1 (bs-1353R), HRP-conjugated secondary antibodies were purchased from Bioss Biotechnology Co., Ltd. (Beijing, China).

Techniques: Transfection, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot

Effect of high glucose and LOX-1 silencing on HNF4α localization in HLSECs. HLSECs were treated with HG, HG + LV-LOX-1 and HG + LV-CON for 72h. The fixed cells were stained with HNF4α primary antibody and overlaid with goat anti-rabbit secondary antibody (green) and then with DAPI (blue) (n = 3 independent experiments). The scale bar in each image represents 50 μm.

Journal: Heliyon

Article Title: LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs

doi: 10.1016/j.heliyon.2022.e12385

Figure Lengend Snippet: Effect of high glucose and LOX-1 silencing on HNF4α localization in HLSECs. HLSECs were treated with HG, HG + LV-LOX-1 and HG + LV-CON for 72h. The fixed cells were stained with HNF4α primary antibody and overlaid with goat anti-rabbit secondary antibody (green) and then with DAPI (blue) (n = 3 independent experiments). The scale bar in each image represents 50 μm.

Article Snippet: Antibodies against AMPK (bs-1115R), p-AMPK (bs-14318R), HNF4α (bs-3828R), p-HNF4α (bs-4001R), LC3 (bs-8878R), beclin-1 (bs-1353R), HRP-conjugated secondary antibodies were purchased from Bioss Biotechnology Co., Ltd. (Beijing, China).

Techniques: Staining